The present work describes a simple, rapid and reproducible reverse phase high performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of clindamycin phosphate (CLINDA) and nicotinamide (NICO). The separation was achieved on a hypersil BDS C18 (250 mm X 4.6 mm i.d., 5 μm particle size) with an isocratic system of Phosphate buffer (pH-3.5): methanol in the ratio of 60:40 v/v. The mobile phase at a flow rate of 1.0 ml/min, injection volume 20 μl and wavelength of detection used was 210 nm. The retention time for nicotinamide and clindamycin phosphate were obtained as 4.053±0.11 min and 5.730±0.1 min, respectively. The linearity of the proposed method was investigated in the range of 5-15 µg/ml and 20-60 µg/ml for clindamycin phosphate and nicotinamide, respectively. Correlation coefficients were 0.998 and 0.999 for clindamycin phosphate and nicotinamide, respectively. The developed method was validated as per ICH guideline, for its accuracy, precision, LOD and LOQ and the results were found to be satisfactory, thus the method is specific, rapid and simple with good sensitivity for estimation of clindamycin phosphate and nicotinamide. These analytical methods are also applicable in ordinary laboratories. It can also be adopted for quality control tests for these drugs in cream formulation.
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